Only canon

Rick Sanchez is Pansexual
BMO is non-binary
Spongebob (like a actual sponge) is intersex
Korra is Bisexual
Mr. Garrison is Transgender
Shiro from Voltron is Gay
Luz Noceda is bisexual
Jackie Lynn Thomas is Pansexual
Marceline is Bisexual
Luna Loud is Lesbian
Sheriff Blubs and Deputy Durland are Gay
Velma Dinkley is Bisexual
and Sailor Uranus is gender-fluid

Shiro is gayyy???????

yes

Wish i still had netflix so I could watch voltron again

Spongebob is also Asexual

Raine Whispers (The Owl House) non-binary
Kris Dreemurr (Deltarune) non-binary
Noelle Holiday (Deltarune) sapphic
Susie (Deltarune) probably also sapphic
Undyne (Undertale/Deltarune) sapphic
Alphys (Deltarune/Undertale) sapphic

Deltarune really likes their lesbians

oh, definitely
Rudy and Asgore also seem a little bit fruity

Amity Blight is lesbian

Adora lesbian

Catdira catota catora!

*catdora

I see someone has seen my meme.

i only know anime and a few cartoons so my list will mostly be anime-

waylon smithers (the simpsons) is gay
ryuji ayukawa (blue period) is a crossdressing bi male (i think-)
osana najimi (komi can't communicate) is (biologically male) non-binary (although due to some translation complications sometimes in the subtitles najimi will be referred to as he)
richard plantagenet (requiem of the rose king, will air next year) is an intersex male
crona (soul eater) is gender non-comforming but experiences the same translation complications for sub/dub as najimi
ren yamai (komi can't communicate) is (probably based on her behavior and her love for komi) lesbian
andd like all the main characters in every yaoi or yuri anime are gay/lesbian/other- cuz that's exactly wut yaoi/yuri is

NAJIMI MY LOVE i started the show two days ago and i love najimi sm
also Yamai does have a (rather obsessive) crush on Komi

yeah ik she kidnapped tadano how is that not yandere-