Markiplier is often seen doing and speaking silly things, and in a few YouTube clips, he even laughed and acted like a girl, so some of his fans thought him gay. But almost every YouTuber who makes gameplay videos talks silly and does silly things.
Since Markiplier has a huge fan base, he has many haters as well. They spread rumours like Fischbach is gay, Fischbach is bisexual, while the truth is Mark Fishbach is straight.